Fig. 4

IGF-1 ameliorates the FPI-induced increase in immature neurons in the adult hippocampus. Immature neurons in the hippocampal dentate gyrus were assessed by stereological quantification of the number of DCX-labeled cells at 3 DPI (a–c). The results show that FPI increased the number of DCX + cells (p = 0.051), and this was mitigated by IGF-1 treatment (d). The number of proliferative cells in the dentate gyrus (DG) was assessed by quantifying the number of Ki67-labeled cells (e–g) in the hilus (h), granule cell layer (GCL), and subgranular zone (SGZ). No significant differences in the number of Ki67 + cells in the hippocampus were identified at 3 DPI (h). Scale bar in c = 20 µm for (a–c). Data are represented as Mean ± SEM; n = 3–4 per group